We mist-netted bats to test the efficacy of the US Fish and Wildlife Service Indiana Bat Protocol (IBP), which recommends monitoring nets every 20 min. We continuously videotaped mist nets while monitoring and removing bats every 20 min. We recorded 157 bats on video that approached within 10 m of the mist net. Thirty-six bats (23.1%) avoided the net, 79 (50.0%) were caught and collected, and 42 (26.9%) were caught but escaped before we returned to check the net. We recommend that researchers check mist nets at intervals ≤ 10 minutes to potentially increase capture rates by ≥ 25%.